南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测          下载免费PDF全文

王飞  丛柏林  张朝晖  杨黄浩 《极地研究》2020,32(1):68-81

研究了南极罗斯海地区沉积物中可培养微生物多样性,并检测了菌株胞外酶活性,为开发利用南极资源奠定基础。采用Zobell2216E培养基、土豆培养基(PDA)及稀释平板划线法分离并获得纯培养的细菌和真菌,分别通过16SrDNA、18S/ITS序列鉴定分析了可培养微生物的多样性和系统发育。从南极罗斯海沉积物样品中获得78株细菌和54株真菌,细菌、真菌的优势菌株分别为嗜冷杆菌属(Psychrobacter)、枝孢属(Cladosporium);78株细菌菌株中有2株与模式菌株的16SrDNA基因序列相似性小于97%,可能是潜在的细菌新物种。细菌API20NE生理生化和真菌产胞外酶活性结果表明,获得的细菌和真菌大多数可检测到胞外水解酶活性。本研究丰富了南极可培养微生物资源,为南极罗斯海地区可培养微生物的多样性研究以及南极微生物资源的应用研究奠定了基础。  相似文献   

Simulating cross-sectional geometry of the main channel in response to changes in water and sediment in Lower Yellow River     

Wang  Yanjun  Wu  Baosheng  Zhong  Deyu 《地理学报(英文版)》2020,30(12):2033-2052

Journal of Geographical Sciences - To understand the non-equilibrium morphological adjustment of a river in response to environmental changes, it is essential to (i) accurately identify how past...  相似文献   

Upper ocean high resolution regional modeling of the Arabian Sea and Bay of Bengal     

Suneet Dwivedi  Alok Kumar Mishr  Atul Srivastava 《海洋学报(英文版)》2019,38(5):32-50

In this paper, effort is made to demonstrate the quality of high-resolution regional ocean circulation model in realistically simulating the circulation and variability properties of the northern Indian Ocean(10°S–25°N,45°–100°E) covering the Arabian Sea(AS) and Bay of Bengal(BoB). The model run using the open boundary conditions is carried out at 10 km horizontal resolution and highest vertical resolution of 2 m in the upper ocean.The surface and sub-surface structure of hydrographic variables(temperature and salinity) and currents is compared against the observations during 1998–2014(17 years). In particular, the seasonal variability of the sea surface temperature, sea surface salinity, and surface currents over the model domain is studied. The highresolution model's ability in correct estimation of the spatio-temporal mixed layer depth(MLD) variability of the AS and BoB is also shown. The lowest MLD values are observed during spring(March-April-May) and highest during winter(December-January-February) seasons. The maximum MLD in the AS(BoB) during December to February reaches 150 m (67 m). On the other hand, the minimum MLD in these regions during March-April-May becomes as low as 11–12 m. The influence of wind stress, net heat flux and freshwater flux on the seasonal variability of the MLD is discussed. The physical processes controlling the seasonal cycle of sea surface temperature are investigated by carrying out mixed layer heat budget analysis. It is found that air-sea fluxes play a dominant role in the seasonal evolution of sea surface temperature of the northern Indian Ocean and the contribution of horizontal advection, vertical entrainment and diffusion processes is small. The upper ocean zonal and meridional volume transport across different sections in the AS and BoB is also computed. The seasonal variability of the transports is studied in the context of monsoonal currents.  相似文献   

南海东北部内潮的模态结构和传播     

刘倩  谢晓辉  尚晓东  陈桂英  汪宏 《海洋学报(英文版)》2019,38(9):12-23

The evolution of energy, energy flux and modal structure of the internal tides(ITs) in the northeastern South China Sea is examined using the measurements at two moorings along a cross-slope section from the deep continental slope to the shallow continental shelf. The energy of both diurnal and semidiurnal ITs clearly shows a～14-day spring-neap cycle, but their phases lag that of barotropic tides, indicating that ITs are not generated on the continental slope. Observations of internal tidal energy flux suggest that they may be generated at the Luzon Strait and propagate west-northwest to the continental slope in the northwestern SCS. Because the continental slope is critical-supercritical with respect to diurnal ITs, about 4.6 kJ/m~2 of the incident energy and 8.7 kW/m of energy flux of diurnal ITs are reduced from the continental slope to the continental shelf. In contrast, the semidiurnal internal tides enter the shelf because of the sub-critical topography with respect to semidiurnal ITs.From the continental slope to the shelf, the vertical structure of diurnal ITs shows significant variation, with dominant Mode 1 on the deep slope and dominant higher modes on the shelf. On the contrary, the vertical structure of the semidiurnal ITs is stable, with dominant Mode 1.  相似文献   

基于IceBridge数据的南极别林斯高晋海的海冰厚度研究     

张建  杨元德  杨全明  汪楚涯 《冰川冻土》2020,42(2):295-306

海冰变化与全球气候、 生态系统和人类活动密切相关， 海冰厚度是海冰变化研究的重要参数之一。全面立体高精度观测海冰厚度的最有效手段是航空遥感， 而冰桥计划（IceBridge）是当前南北极最大的航空遥感工程。基于2009 - 2014年冰桥计划的激光雷达高程数据和数字测图系统相机光学影像对南极别林斯高晋海的海冰厚度进行研究， 并结合降雪量等气象数据探讨该区域海冰厚度变化的原因。研究发现该海域的海冰厚度在2009 - 2014年间整体呈微弱增长趋势（0.07 m·a^-1）， 但是在95%置信水平下不具有显著性。2009 - 2011年呈现先增加后减少的大幅度变化， 其中2010年达到极大值2.42 m， 之后开始缓慢增加。海冰厚度的年际变化与降雪和近地表温度等气象要素相关， 二者相比较而言降雪为主要影响因素。  相似文献   

持续大流量过程对黄河内蒙古河段河道塑形能力的分析与思考     

方立  冯缠利  郑宝旺  沈国庭 《地下水》2020,(1):152-154,266

2018年伏秋汛期黄河流域上游持续来水,为保证2108-2019年度黄河流域凌汛期安全,黄河流域重点水库进行了大流量持续下泄。以2018年9月的实际数据为基准,通过对重点水库实际日均出入库调度情况,内蒙古河段的重点水文站实际日均流量过程和三个年份汛期大断面套绘成果对比分析研究,可以得出水库大流量持续下泄对内蒙古河段河道塑形能力起到了关键性作用,有效的提高了主槽过流能力,河段最小平滩流量得到一定的恢复,对下一步研究黄河流域河道过流能力提供了有利的数据支撑和参考价值。  相似文献   

GPM和TRMM卫星日降水数据在黄河源区的适用性分析     

刘兆晨  杨梅学  王学佳  程立真 《冰川冻土》2020,42(2):575-586

通过评估GPM计划三种日降水产品（IMERG-E、 IMERG-L和IMERG-F）和TRMM卫星、 两种日降水产品（TMPA 3B42和TMPA 3B42RT）在黄河源及其周边区域38个台站的适用性， 探究了五种产品探测精度和海拔高度及雨强的相关关系， 结果表明： 在与实测资料的一致性和偏差方面， GPM卫星产品要全面优于TMPA产品。在TRMM卫星产品中， 3B42产品明显优于3B42RT。五种产品的相关系数均表现出明显的从东南到西北递减的趋势， 均方根误差北部普遍低于南部。IMERG产品的探测率（POD）和探测成功率（CSI）都要普遍高于TMPA产品， 而误报率（FAR）则是TMPA 产品更低， 表现更好。五种产品均在个别台站出现了严重误报的情况， 这些台站主要分布在研究区的西北部。IMERG三种产品对于海拔高度的依赖程度具有很强的一致性， 而3B42RT产品对海拔高度几乎没有依赖。除3B42RT产品外， 其余四种产品的偏差均随雨强的增加而增大。在探测率方面， IMERG产品对小雨、 中雨和大雨的探测能力均优于TMPA产品。  相似文献   

Numerical Study on Tidal Mixing in the Bohai Sea     

Yifei Zhao  Ting Yu  Hu Wang 《Marine Geodesy》2019,42(1):46-63

The spatial and temporal variability of tidal mixing in Bohai Sea is studied using a numerical approach. In calculating tidal mixing, accurate barotropic tidal current is obtained via a harmonic analysis package utilizing the simulated current output from a high-resolution regional ocean model. And a “small-scale” roughness map is adopted to describe the detailed topographic features of Bohai Sea. It is shown that the tidal mixing estimated in Bohai Sea is much higher than the level of global background, and fluctuates considerably at some regions within a single day. In Liaodong Bay, Bohai Bay and Bohai Strait, the mixing varies greatly, with the peak value of O (10^?2) m² s^?1. The order of magnitude of mixing in Laizhou Bay is about O (10^?5～10^?3) m² s^?1. Mixing with background level of O (10^?5) m² s^?1 only appears in central area. Result also shows that rough topography plays relatively a more important role than tidal current in enhancing diapycnal mixing in Bohai Sea. The distributions of tidal mixing in selected sections reveal that the vertical stratification in Bohai Sea is not obvious, generally renders a barotropic structure.  相似文献   

南海盐致环流对台风降水的响应特征分析     

刘娜  王桂华  巩远发  刘磊 《热带海洋学报》2019,38(3):13-21

本文采用多源卫星遥感数据通过统计分析的方法研究了17年间(2000—2016年)南海夏季(6—9月)台风对该海域降水、淡水通量的贡献及其可能导致的环流异常。主要结论如下: 1) 台风是南海中北部降水的重要影响因子, 可导致日平均降水量增加12mm, 约占南海夏季日平均降水(25mm·d ^-1)的一半, 且西北太平洋台风和南海“土台风”产生的降水分布存在显著的区域和强度差异; 2) 夏季, 南海由淡水通量引起的盐致环流表现为以海南岛东南部海域为中心的弱气旋式, 其流量量级约为-0.15Sv, 约占同期风生环流流量(约为-1.5Sv)的10%; 3) 夏季, 台风带来的降水使得南海中北部的气旋式盐致环流增强, 且西北太平洋台风降水导致的淡水通量变化引起的盐致环流强度要强于南海“土台风”。  相似文献   

Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments     

Gozari Mohsen  Bahador Nim  Jassbi Amir Rez  Mortazavi Mohammad Seddiq  Hamzehei Sam  Eftekhar Ebrahim 《海洋学报(英文版)》2019,38(12):84-90

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.  相似文献